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cd47(b6h12.2)  (Biotium)


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    Biotium cd47(b6h12.2)
    Cd47(b6h12.2), supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf+640r+conjugated+mouse+anti+human+cd47+ab/CD47(B6H12%2E2)/custom%40bnc000437-100%4040334763
    Average 92 stars, based on 174 article reviews
    cd47(b6h12.2) - by Bioz Stars, 2026-11
    92/100 stars

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    Incubation:

    Article Title: Rituximab-IgG2 is a phagocytic enhancer in antibody-based immunotherapy of B-cell lymphoma by altering CD47 expression
    Article Snippet: .. Next, the cells were incubated overnight at 4°C with 3 μg/mL of CF ® 640R-conjugated mouse anti-human CD47 Ab (clone B6H12.2, BNC400437-100, Biotium, Fremont, CA, USA) and 1× Hoechst 33342 Ready FlowTM Reagent (R37165, Thermo Fisher Scientific) prepared in 1% BSA in PBS. .. Stained cells were then washed, centrifuged, re-dispersed in 20 μL of deionized water, and added onto Superfrost plus microscope slides (J1800AMNZ, Thermo Fisher Scientific) for air-drying.

    Article Title: Rituximab-IgG2 is a phagocytic enhancer in antibody-based immunotherapy of B-cell lymphoma by altering CD47 expression.
    Article Snippet: .. Next, the cells were incubated overnight at 4°C with 3 mg/mL of CF®640R-conjugated mouse anti-human CD47 Ab (clone B6H12.2, BNC400437-100, Biotium, Fremont, CA, USA) and 1× Hoechst 33342 Ready FlowTM Reagent (R37165, Thermo Fisher Scientific) prepared in 1% BSA in PBS. .. Stained cells were then washed, centrifuged, re-dispersed in 20 mL of deionized water, and added onto Superfrost plus microscope slides (J1800AMNZ, Thermo Fisher Scientific) for air-drying.

    other:

    Article Title: Rituximab-IgG2 is a phagocytic enhancer in antibody-based immunotherapy of B-cell lymphoma by altering CD47 expression
    Article Snippet: To analyze CD47 expression, RTX or STR-treated Granta-519 cells were washed with cold flow staining buffer and fixed with 50 μL of fixation buffer (cat. no. 88-8824, Thermo Fisher Scientific) for 15 min on ice.



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